Composite

Part:BBa_K324050:Design

Designed by: Ethan Richman   Group: iGEM10_Brown   (2010-10-25)

TrpR-RBS-TetR-Term-pTetR-RBS-CI-RBS-AraC-RBS-Mnt-Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because there are illegal SpeI sites in the Trp promotor, all assembly had to be done with RFC 21 standard (bgl bricks). Thus, we had to add the appropriate bgl-brick sites to all parts we worked with. See [http://2010.igem.org/Team:Brown/Project/LRC_overview our team page] for a more in depth description.

Source

Part:BBa_K191005 for TrpR-RBS-TetR-Term-pTetR (PCR to isolate this segment) Part:BBa_B0034 for sequence of RBS (added RBS by PCR to CI, AraC, and Mnt)


References